Fig. 2. FIR-induced EMT signaling proceeds through Akt à Src à Erk pathways. HCT116 p53⁺/⁺ cells were irradiated with 2 Gy per day for 3 days in the presence or absence of kinase inhibitors: (A) Akt inhibitor LY294002, (B) Src inhibitor, PP1, and (C) Erk inhibitor, PD98059 at indicated concentrations treated 1 h prior to the last irradiation. Expression levels of signaling molecules and EMT markers were analyzed by Western blot as described in Fig. 1. A representative blot is shown. (D) Quantitative data obtained from the densitometry analysis of multiple blots are expressed as described in Fig. 1.